Journal: Stroke
Article Title: Pharmacological Induction of Heme Oxygenase-1 by a Triterpenoid Protects Neurons Against Ischemic Injury
doi: 10.1161/strokeaha.111.647420
Figure Lengend Snippet: Figure 5. CDDO-Im protects hippocampal CA1 neurons against global ischemia via HO-1 in rats. A, A representative EEG re- cording in a rat undergoing global cerebral ischemia, showing rapid isoelectricity after the onset of ischemia. B, H&E stain of CA1 regions and quantitative analysis of viable neurons after global ischemia in rats. a, sham; (b) vehicle-treated ischemia; (c) CDDO-Im-treated ischemia; and (d) CDDO-Im- and tin protoporphyrin IX (Sn- PPIX)-treated ischemia. Bar50 m; n8, *P0.05 versus sham, #P0.05 versus vehicle-treated ischemia, and &P0.05 versus CDDO-treated ischemia. C, PANT stain of CA1 regions and quantitative anal- ysis of dead neurons. a, sham; (b) vehicle- treated ischemia; (c) CDDO-Im-treated ischemia; and (d) CDDO-Im- and Sn-PPIX- treated ischemia. Bar100 m; n8, *P0.05 versus sham, #P0.05 versus vehicle-treated ischemia, and &P0.05 versus CDDO-treated ischemia. D, West- ern blots of HO-1 in CA1, demonstrating pre-existence of HO-1 in CA1 after CDDO-Im treatments. n3, **P0.01 ver- sus sham, ##P0.01 versus sham and vehicle-treated ischemia. S indicates sham; V, vehicle-treated; CD, CDDO-Im- treated; CDDO-Im, 2-cyano-3,12 dioxooleana-1,9 dien-28-oyl imidazoline; HO-1, heme oxygenase-1; EEG, electroen- cephalography; H&E, hematoxylin and eosin.
Article Snippet: Electroencephalography (EEG) was recorded to ensure isoelectricity at the onset of ischemia using a PowerLab system (ADInstruments, Colorado Springs, CO).
Techniques: Staining